Taihu Goose Artificial Insemination Technology
The technology of artificial insemination in Chinese geese started late, but this technology has gradually gained importance with the intensive production of the water poultry industry. In the geese industry, where the market is increasingly competitive, reducing the cost of production, especially the breeding cost of breeding geese, is particularly important for increasing economic efficiency. Artificial insemination can increase the proportion of breeding geese by 3 times, which means that 3/4 of the goslings can be reduced, greatly improving the utilization rate of excellent male gooses, and reducing the transmission of genital diseases during natural mating. Improve the fertilization rate of eggs and increase the economic benefits of geese production.
First, the selection and training of male goose
Fifteen to 30 days prior to fertility training, spare-breeding male geese and female geese with robust physique and vigorous libido are reared separately. When training, cut off the male goose cloacal around the diameter of 5 ~ 7 cm? feathers, in accordance with the method of massage sperm extraction method, every day at regular intervals only on mature and healthy male geese training 1 to 2 times, for 5 to 7 days. During the training process, the male geese with weak sexual reflexes, poorly developed penis, less than fine sperm, or with less sperm volume and poor quality should be eliminated in time.
Second, mining technology
The goose picking method works best with back-to-back massage. The operation method is as follows: The spermatozoa person's left palm is close to the back of the goose and strokes 4 to 5 times from the base of the wing to the tail. Then the left hand slightly squeezes the root of the goose tail while the right thumb and index finger rhythmically massage the abdomen. Behind the soft part, and gradually massage and squeeze the goose cloaca ring on both sides. When the male goose penis is fully erected and erupted, it is necessary to slightly squeeze the back side of the cloaca so that the semen can be discharged smoothly, and the spermatozoa will be collected with the spermatrices facing upward.
Third, the characteristics of semen
Normal, non-polluting semen is a milky, opaque liquid that, when mixed with blood, is pink and contaminated with faeces is yellow-brown, and cannot be used for artificial insemination. The average ejaculation amount of male goose is generally 0.1 to 1.38 milliliters. The amount of ejaculate varies greatly depending on the age, season, individual differences, and proficiency of the sperm extractor. Generally, the amount of ejaculate is selected to be large. Stable male geese are used for breeding. The collected semen was placed in a constant temperature chamber at about 37°C or on a constant temperature plate. The sperm of the elite male goose was vortexed and rolled at a microscope of 200-400 times. The sperm motility was 0.8 grade. .7 ~ 0.9 grade, sperm motility can be observed under the microscope to the number of sperm moving straight forward; sperm density of up to 1 billion / ml, the original semen can be measured with 3% sodium chloride After the solution was diluted 200-fold, the sperm density was calculated using a hemacytometer. Checking the quality of semen can help improve the fertilization rate of the eggs and the quality of the semen of each species of goose, so as to make appropriate adjustments, and provide scientific basis for the correct feeding and management of male geese.
Fourth, the dilution and preservation of semen
The role of semen dilution is mainly to expand the semen, increase the amount of insemination, supplement nutrients and protective substances, reduce the harm of lactic acid to sperm, thereby prolonging the survival time of sperm in vitro. At present, 0.9% of normal saline is used in production, or 300 to 500 units/ml of penicillin or streptomycin is added to dilute the semen. The ratio between the mixed semen and the diluent can be diluted 1:1 to 3, and the insemination effect is good. The general requirement is that the number of live sperm after dilution is 300-400 million/ml.
Fifth, insemination technology
1. Insemination equipment: Use 1 ml tuberculin syringe.
2. Insemination method: It can be divided into five steps. The first step: fix the goslings ready for insemination. The assistant grasps the root of the female goose wing in both hands to lie on the ground or on a platform about 70 cm high. The second step is to gently press the lower edge of the cloaca to make the cloaca open. Before insemination, cut the feathers around the anus. After washing the anus with a cotton ball soaked in saline, the inseminat's left four fingers close together and put the tail feathers to the side. The thumb is close to the lower edge of the cloaca and pressed down gently. , so that the cloacal open. Step 3: Insert the spermator. At this time, the inseminat's right hand slowly inserts the semen with semen into the left lower part of the cloaca. When you feel that there is no obstruction, it means that the insemination device has accurately entered the vaginal region, generally 5 to 7 cm deep. Step 4: Enter semen. At this time, the inseminator stabilizes the spermatophore and slowly injects the semen. Step 5: Pull out the spermator. After the completion of insemination, gently pull out the anaesthetic device and at the same time loosen it to relieve the pressure. The vaginal opening can be slowly retracted to the cloaca and the geese can be gently placed on the ground.
3. Insemination time: better from 4:00 to 6:00 in the afternoon. Because the laying time of the mother goose is mostly in the morning or in the morning, most of the eggs in the womb do not form hard shell eggs. At this time, artificial insemination of gooses is conducive to the operation of sperm, thereby increasing the fertilization rate of the breeding eggs.
4. Number of insemination and insemination: Goose sperm lived in the fallopian tube of the goose and maintained a certain fertilization capacity for up to 10 days. Practice has proved that it is generally good to inseminate once every 5 to 6 days; each insemination is 0.1 ml, 0.2 ml for the first time, and the fertilization rate of the 5 days of egg collection from the 2nd day can be achieved. More than 80%.
VI. Precautions in the process of artificial insemination
1. The equipment required for sperm collection and insemination must be sterilized by autoclaving. Dilutions must be sterilized in a pressure cooker at 9.80710 to 14.710 Pb for 30 minutes and then left for use after being naturally cooled.
2. Stop watering for 3 to 5 hours before harvesting to reduce fecal contamination of semen.
3. Semen collection personnel must be fixed, because each person's gestures and strength are different, replacement personnel can easily cause a male goose stress and affect the quality of semen; massage stimulation should be appropriate, excessive stimulation can easily cause defecation and urine production Too much transparent liquid.
4. It is strictly forbidden to smoke during the sperm extraction and dilution process, and to avoid strong light and large temperature difference. The time from semen collection to the end of insemination should not exceed 90 minutes, so as not to affect the insemination effect.
5. In the insemination, the air bubbles in the spermatrices should be excluded. Otherwise, the input semen overflows, affecting the fertilization rate of the egg.
6. Insemination site should be moderate, in order to insert cloaca 5 ~ 7 cm is appropriate, too easy to spill over too shallow, too deep will affect the hatching effect of hatching eggs, increase dead embryos.
7. Goslings suffering from reproductive tract inflammation and other diseases should not be inseminated and should be treated in time for isolation. Every time you lose a goose, it is best to wipe the inseminator with an alcohol swab to prevent cross-infection.
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