Diagnosis and Prevention of Newcastle Disease of Guinea Fowl
In September 2005, a farmer in the Totaizi Village of the Jilin Economic Development Zone purchased 400 3-day-old young chicks. The chicks were in good spirits and had normal appetite. No vaccinations were given during this period. However, after 10 days of age, one after another, the onset was mainly gastrointestinal and respiratory symptoms. The incidence rate was as high as 40%, and the mortality rate was 15%.
1 Clinical symptoms
The initial temperature of the diseased chicken is as high as 43-44°C, and the appetite is diminished or discarded. There is a feeling of thirst. Apathetic, reluctant to walk, drooping neck or wings drooping, eyes half open or closed, cocks and flesh darkened red or dark purple, cough, dyspnea, mucous rhinorrhea, full of liquid contents in the hernia sac There is a lot of sour liquid that flows out of the mouth when poured up. Feces are thin green or yellow-white. The body temperature of the chick with a longer duration of illness decreased, and the finfall could not be restored, and the leg collapsed and fell into a coma and died. The course of disease is usually 1 to 3 days.
2 pathological examination
A total of 10 diseased chickens were co-dissected. The pathological changes were: whole body mucous membrane and serosa hemorrhage, lymphatic system swelling, hemorrhage and necrosis. There is a lot of white mucus in the oral cavity. The phlegm is filled with a thin and sour liquid. The mucous membrane of the glandular stomach is edema, and there is a distinct bleeding point between the nipple and the nipple. Small intestine, cecum, and rectal mucosa have bleeding spots of varying sizes. There is a small, needle-like bleeding spot on the crown of fat.
3 laboratory diagnosis
3.1 Bacterial culture
The diseased spleen was used as a contact patch and no bacteria were detected by Gram staining. The diseased spleen tissue was inoculated on agar medium and blood culture medium and incubated in a 37°C incubator for 24 hours without bacterial growth.
3.2 Chicken embryo inoculation
Take the diseased spleen, and use a sterile saline solution at a ratio of 1:10 to prepare a suspension and apply double-antibiotic treatment. After 4 to 6 hours in a refrigerator at 4°C, centrifugation at 3000r/min for 15 minutes, 0.2 mL of the supernatant was inoculated on 10 day-old SPF chicken embryos, placed in a thermostat and humidity chamber at 37°C for 5 days. All chicken embryos died. Allantoic fluid was tested for HA and the results were positive and could be inhibited by standard DNV antiserum. Combined with epidemiology, clinical symptoms, and pathological necropsy, it can be determined that the disease is Newcastle disease.
4 Control measures
4.1 Dead chicks and feces buried deep and harmless. The site was disinfected with 2% fire alkali; the indoor and cages were sprayed with dual-quaternary ammonium iodide 400-600 times. Incoming and outgoing personnel and vehicles are strictly sterilized. Diseases, chickens and chicks were fed separately.
4.2 Intramuscular injection of Newcastle disease high yolk immunoglobulin antibody produced by the Veterinary Research Institute of Jilin Province, 0.5 mL/body for 3 days.
4.3 Groups of chicks were immediately immunized with drinking water from IV-type attenuated seedlings (Lasota) for multiple drinking or multiple drinking.
4.4 use Qingdai bulk spices, chlortetracycline or Lome star drinking water for 3 to 4 days, while adding supplemental ~ 14 or electrolytic multidimensional.
After the above comprehensive prevention and control measures, the condition has been controlled.
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