Ion absorption in the recovery of swell pressure of Arabidopsis root epidermal cells after osmotic stress

Ion absorption in the recovery of swell pressure of Arabidopsis root epidermal cells after osmotic stress

Note: osmotic stress induced cell turgor and kinetic changes in K + ion current. The hypertonic treatment resulted in a rapid decrease in turgor pressure, while the K + inflow increased, the turgor pressure recovered at 40 min, and the K + influx decreased.

Improving crop drought resistance is a long-standing challenge for plant physicists and agricultural biotechnologists. In recent years, increasing crop drought work focuses on transgenic studies, but there is no description reported GM crops in the field that can significantly improve the drought resistance of crops.

Hyperosmotic stress (drought) causes a large amount of inorganic ions to enter plant cells, but direct evidence of cell turgor recovery has been lacking. Scientific non-invasive micro-testing technology Simultaneous determination of ion flux and turgor pressure in root epidermal cells of Arabidopsis thaliana under osmotic stress was observed by pressure probe technique. It was found that after hypertonic stress (100/100 mM mannitol/sorbitol treatment), cell swell pressure was observed. 0.65 MPa quickly drops to 0.25 MPa. The turgor recovery was initiated within 2-10 min after treatment, accompanied by a large increase in K ⁺ , Cl ^- and Na ⁺ absorption. The turgor pressure of most cells recovered by 90% within 40-50 min after stress. Combined with voltage clamp and non-damage micro-test techniques, the process of swell recovery is due to the regulation of voltage-gated K ⁺ transporters on the plasma membrane.

This study directly obtained changes in cellular ion fluxes through non-invasive microtesting techniques, and this change was associated with changes in cell turgor pressure, providing evidence for understanding the mechanisms of drought. In future studies, researchers can use other osmotic adjustment materials to study the effects of ion flow and osmotic adjustment using non-invasive micro-test techniques to screen for substances that improve plant drought resistance.

Key words: hyperosmotic stress; cell turgor; non-invasive micro-test technique

References: Shabala SN, Lew RR. Plant Physiology , 2002, 129: 290–299.

Full text download