High pressure steam sterilizer operation steps

High pressure steam sterilizer operation steps

Clicks: 4410 Release time: 2016/6/7

Steps

1. First the inner layer was extracted tub sterilization, and then an appropriate amount of water was added to the outer pot, so the water phase and the triangular shelf level is appropriate.

2. Put back the sterilization bucket and load the items to be sterilized. Be careful not to pack too much, so as not to hinder the flow of steam and affect the sterilization effect. Do not contact the barrel end of the flask and the end of the tube to prevent the condensate from drenching the paper at the mouth and penetrating into the tampon.

3. Capped, sterilized and inserted into the exhaust slot inner tub lid exhaust tube. Then tighten the two bolts at the same time in a symmetrical manner to make the bolts tight and not leaking.

4. Using electric or gas heating, and simultaneously open the exhaust valve, to boil water to eliminate air cooling pot. After the cold air is completely drained, close the exhaust valve so that the temperature inside the pot gradually increases as the steam pressure increases. When the pressure in the pot rises to the desired pressure, the heat source is controlled to maintain the pressure for the required time. This experiment was sterilized with 1.05 kg/cm 2 , 121.3 ° C for 20 minutes.

5. After the time required for sterilization, turn off the power or turn off the gas, let the temperature inside the sterilizer drop naturally. When the pressure of the pressure gauge drops to 0 , open the vent valve, loosen the bolt, open the lid, and take out the sterilized items. If the pressure does not drop to 0, open the exhaust valve, the pressure in the pot will suddenly drop, so that the medium in the container will be flushed out of the flask mouth or the test tube mouth due to the imbalance of internal and external pressure, causing the cotton plug to contaminate the medium. Pollution.

6. The sterilized medium taken out was placed in a 37 ° C incubator for 24 hours, and it was checked if no bacteria were grown.

Steps

1. First, remove the inner layer sterilization barrel, and then add an appropriate amount of water to the outer layer pot to make the water surface and the triangle shelf level.

2. Put back the sterilization bucket and load the items to be sterilized. Be careful not to pack too much, so as not to hinder the circulation of steam and affect the sterilization effect. Do not contact the barrel end of the flask and the end of the tube to prevent the condensate from drenching the paper at the mouth and penetrating into the tampon.

3. Cap and insert the exhaust hose of the cover into the exhaust groove of the inner sterilization tank. Then tighten the two bolts at the same time in a symmetrical manner to make the bolts tight and not leaking.

4. Heat with an electric stove or gas and open the exhaust valve at the same time to boil the water to remove the cold air from the pot. After the cold air is completely drained, close the exhaust valve so that the temperature inside the pot gradually increases as the steam pressure increases. When the pressure in the pot rises to the desired pressure, the heat source is controlled to maintain the pressure for the required time. This experiment was sterilized with 1.05 kg/cm2 at 121.3 °C for 20 minutes.

5. After the time required for sterilization, turn off the power or turn off the gas, let the temperature inside the sterilizer drop naturally. When the pressure of the pressure gauge drops to 0, open the vent valve, loosen the bolt, open the lid, and take out the sterilized items. If the pressure does not drop to 0, open the exhaust valve, the pressure in the pot will suddenly drop, so that the medium in the container will be flushed out of the flask mouth or the test tube mouth due to the imbalance of internal and external pressure, causing the cotton plug to contaminate the medium. Pollution.

6. The sterilized medium taken out was placed in a 37 ° C incubator for 24 hours, and it was checked if no bacteria were grown.

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