MOPS Sodium Salt: An Analysis of Its Uses and Recommendations in Biochemistry and Molecular Biology
MOPS sodium salt, often utilized in biochemical and molecular biology research, serves as an essential biological buffer. These buffers play a critical role in maintaining stable pH levels in solutions by neutralizing acids or bases, thereby preventing significant fluctuations in acidity or alkalinity when these substances are introduced.
Key Applications
MOPS sodium salt (CAS 71119-22-7), chemically known as 3-(N-morpholino)propanesulfonate, appears as a white crystalline powder under standard conditions and exhibits high solubility in water. Its optimal working pH range lies between 6.5 and 7.9, making it indispensable in various fields such as protein extraction, RNA isolation via electrophoresis, and bacterial cultivation for diagnostic purposes. A substantial portion of our supply finds application in preparing growth media for bacteria and yeast, particularly within food microbiology.
Considerations for Specific Uses
When using MOPS sodium salt, certain factors must be taken into account:
- Concentrations exceeding 20 mM may hinder the growth of eukaryotic cells.
- MOPS has a tendency to bind with metals, nucleic acids, and lipids, which could potentially interfere with experimental outcomes.
- In the presence of glucose, it tends to degrade partially during autoclaving processes.
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Relevant References
Taha, M., Gupta, B. S., Khoiroh, I., & Lee, M. J. (2011). Interactions of Biological Buffers with Macromolecules: The Ubiquitous “Smart†Polymer PNIPAM and the Biological Buffers MES, MOPS, and MOPSO. Macromolecules, 44(21), 8575-8589.
Carson, S. D., Hafenstein, S., & Lee, H. (2017). MOPS and Coxsackievirus B3 Stability. Virology, 501, 183-187.
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