New Techniques for Stereo Culture of Black Fungus Big Bag
First, the cultivation site can use vegetable greenhouses, free space, balconies, roofs, shade trees and other venues. Requirements close to the water source, good ventilation, away from pollution sources.
Second, the cultivation season, with the local temperature stable at 15-25°C, is the best ear period.
Third, the raw material formula
1. 90% cottonseed husk, 5% bran, 3% corn (2324, 0.00, 0.00%) powder, 1% lime powder, 1% gypsum powder.
2. Corncob powder 80%, bran 10%, corn flour 5%, soybean (4461, 6.00, 0.13%) powder 2%, lime powder 2%, gypsum powder 1%.
Four, bagging bacteria bags can choose 22 cm x 38 cm x 0.04 cm high-density polyethylene cylinder. When loading, try to tighten, do not make the initial gap between the bag and the material. Zhakou important to prevent the formation of water bags and the cultivation failed::
Fifth, steam sterilization using sterilization, use the appropriate specifications of the boiler. Household cultivation can be changed from steam drums to steam generators. That is to say, cut a mouth on the side of the iron drum, and use a pipe with a diameter of 2 cm to insert the other side of the drum from the opening, and weld the opening: the mouth and the steel pipe in close contact. In the opening of the same side of the opening of a different diameter of 4 cm mouth, and a 4 cm diameter short steel tube slightly inserted into the barrel wall, and) F welded tightly. 2 cm pipe for water, 4 cm pipe for exhaust pipe. Use bricks to form a stove, place the bucket on the stove, add water to the bucket, and then heat the fire to generate steam. In the middle of sterilization, add water to ensure the supply of steam.
6. Inoculated vaccination personnel entered the inoculation room. First wipe the hands with 75% alcohol cotton balls. Each group of 3 persons was a group. One person excavated bacteria from the bottle, and 2 people untied the bag and tied the bag. Two or three strains of red jujube-sized bacteria are attached to each end of the bag. - All the bacteria bags should be completely taken over. The middle should not be arbitrarily exposed. Otherwise, it must be re-fumigated and sterilized before operation.
Seven, after the germination management of the bacteria bag into a clean room or shelter from light culture, keep the temperature 25 ~ 28 °C. The first 5 days do not need to be ventilated, the shed can use the method of adding light or shading to increase or decrease the temperature. When the light is increased, the bag pile should be shaded with a black film or straw curtain. 5 days after the appropriate amount of ventilation, to maintain the relative humidity of 70%. After 40 to 50 days of cultivation, the mycelium is fully bagged and transferred to ear management.
Eight, ear management
1. Site management. If the small ear site is in the shed, a small band of 15 cm in width and 15-20 cm in height can be made in the shed. The distance between the bandits is 80 cm. Ears are also made on the outside in this way. For example, on hardened ground ears, a layer of brick can be laid at the above distance.
2. Fungus bag opening. After washing the surface of the bag with 1% potassium permanganate solution or 0.2% Kezhiling solution, use a sharp blade to cut 4-6 "V"-shaped openings at both ends. The upper diameter of the mouth is about 2 cm.
3. Put bacteria bags. After the hatching, the bag is laid flat on the soil. The distance between the bag and the bag is 5-8 cm, and the soil is filled with soil (if the system is hardened, the soil may not fill the soil). In the upper part of the bag is also spread 2 to 3 cm thick layer of soil, and then according to the first layer pendulum to set the second layer of bacteria bags. If you do not fill the soil, put two bamboo pods with a diameter of 2-3 cm on the first bag, and then put a second layer of bacterium bags; in this way, place 6-8 layers in sequence. Finally, a layer of plastic film was placed near the mound to prevent the soil particles from splashing on the ear when the water was sprayed.
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